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22/01/2022 5:18 pm
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Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also, mention the functions of each tool.
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22/01/2022 5:20 pm
1. Restriction endonucleases: for cutting the desired DNA at desired places
2. Gel electrophoresis: for separating the desired DNA fragments
3. Ligase enzyme: for creating recombinant DNA molecule.
4. DNA delivery system: like electroporation, microinjection, gene gun method.
5. Competent host (usually bacteria/yeast): to take up recombinant DNA.