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Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also, mention the functions of each tool.

  

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Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also, mention the functions of each tool.

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1. Restriction endonucleases: for cutting the desired DNA at desired places

2. Gel electrophoresis: for separating the desired DNA fragments

3. Ligase enzyme: for creating recombinant DNA molecule.

4. DNA delivery system: like electroporation, microinjection, gene gun method.

5. Competent host (usually bacteria/yeast): to take up recombinant DNA.

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