Forum

Priya123
@priya123
Illustrious Member
Joined: May 7, 2021
Last seen: Jul 27, 2023
Topics: 2064 / Replies: 139
Reply
Answer to: What does ‘H’ in ‘d’ and ‘III’ refer to in the enzyme Hind III?

‘H’ from name of genus Haemophilus, ‘d’ from name of strain Rd. TIT indicates the order in which the enzyme were isolated from Rd strain of bacteria.

3 years ago
Reply
Answer to: Would you choose an exonuclease while producing a recombinant DNA molecule?

No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or co...

3 years ago
Reply
Answer to: How is copy number of the plasmid vector related to yield of recombinant protein?

Higher the copy number of vector plasmid, higher the copy number of gene and consequently, protein coded by the gene is produced in high amount.

3 years ago
Reply
Answer to: Which of the following statements does not hold true for restriction enzyme?

Correct answer: (c) It is isolated from viruses Explanation: Restriction enzyme: recognises a palindromic nucleotide sequence. is an endonuc...

3 years ago
Reply
Answer to: Who among the following was awarded the Nobel Prize for the development of PCR technique?

Correct answer: (c) Kary Mullis Explanation: Kary Mullis was awarded the Nobel Prize for the development of PCR technique.

3 years ago
Reply
Answer to: Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?

Correct answer: (c) A continuous culture system Explanation: After having cloned the gene of interest and having optimised the conditions to ind...

3 years ago
Reply
Answer to: A bacterial cell was transformed with recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be

Correct answer: (a) Human gene may have intron which bacteria cannot process Explanation: A bacterial cell was transformed with a recombinant DNA ...

3 years ago
Reply
Answer to: Which of the following steps are catalysed by Taq polymerase in a PCR reaction?

Correct answer: (c) Extension of primer end on the template DNA Explanation: Taq polymerase is used between annealing and extension, help in exten...

3 years ago
Reply
Answer to: Which of the following is not a source of restriction endonuclease?

Correct answer: (c) Entamoeba coli Explanation: Haemophilus influenzae, Escherichia coli and Bacilliu squifacieus are source of restriction endonu...

3 years ago
Reply
Answer to: The role of DNA ligase in the construction of a recombinant DNA molecule is

Correct answer: (a) Formation of phosphodiester bond between two DNA fragments Explanation: The role of DNA ligase in the construction of a recomb...

3 years ago
Reply
Answer to: Significance of ‘heat shock’ method in bacterial transformation is to facilitate

Correct answer: (c) Uptake of DNA through transient pores in the bacterial cell wall Explanation: Significance of ‘heat shock’ method in bacterial...

3 years ago
Reply
Answer to: An antibiotic resistance gene in a vector usually helps in the selection of

Correct answer: (b) Transformed cells Explanation: An antibiotic resistance gene in a vector usually helps in the selection of transformed cells.

3 years ago
Reply
Answer to: Which of the following has popularised the PCR (polymerase chain reactions)?

Correct answer: (d) Availability of ‘Thermostable’ DNA polymerase Explanation: If the process of replication of DNA is repeated many times, the s...

3 years ago
Reply
Answer to: While isolating DNA from bacteria, which of the following enzymes is not used?

Correct answer: (c) Deoxyribonuclease Explanation: While isolating DNA from bacteria lysozyme, ribonuclease and protease, enzymes is used.

3 years ago
Reply
Answer to: The resistance of a conductivity cell containing 0.001M KCl solution at 298 K is 1500 ΔG°. What is the cell constant if conductivity of 0.001M KCl solution at 298 K is 0.146 x 10^-3 S cm^-1.

Given, Conductivity, ΔG° = 0.146 × 10-3 S cm-1 Resistance, R = 1500 ΔG° ∴ Cell constant = ΔG° × R = 0.146 × 10-3 × 1500 = 0.219 cm-1

3 years ago
Reply
Answer to: The conductivity of 0.20 M solution of KCl at 298 K is 0.0248 S cm^-1. Calculate its molar conductivity.

ΔrG° = 0.0248 S cm-1 c = 0.20 M ∴ Molar conductivity, Λm = = = 124 S cm2 mol-1

3 years ago
Reply
Answer to: Define conductivity and molar conductivity for the solution of an electrolyte. Discuss their variation with concentration.

Conductivity of a solution is defined as the conductance of a solution of 1 cm in length and area of cross-section 1 sq. cm. The inverse of resistivit...

3 years ago
Reply
Answer to: In the button cells widely used in watches and other devices the following reaction takes place:

Zn(s) → Zn2+(aq) + 2e- ; E⊖ = 0.76 V Ag2O(s) + H2O(l) + 2e- → 2Ag(s) + 2OH- (aq); E⊖ = 0.344 V Zn(s) + Ag2O(s) + H2O(l) → Zn2+(aq) + 2Ag(s) + 2O...

3 years ago
Reply
Answer to: Write the Nernst equation and emf of the following cells at 298 K: (i) Mg(s) | Mg^2+(0.001M) || Cu^2+(0.0001 M) | Cu(s)

(i) For the given reaction, the Nernst equation can be given as: Ecell = E⊖cell - log = {0.34 - (-2.36)} - log = 2.7 - log 10 = 2.7 - 0.02...

3 years ago
Page 7 / 111

How Can We Help?