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Priya123
Priya123
@priya123
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Joined: May 7, 2021
Last seen: Jan 19, 2022
Topics: 0 / Replies: 2086
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Answer to: What is the significance of adding proteases at the time of isolation of genetic material (DNA)?

Role of proteases is to degrade the proteins present inside a cell (from which DNA is being isolated). If the proteins are not removed from DNA prepar...

1 year ago
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Answer to: What does ‘competent’ refer to in competent cells used in transformation experiments?

Competent means bacterial cells, on treatment with CaCl2, are made capable of taking up foreign DNA.

1 year ago
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Answer to: Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.

If the restriction enzymes have more than one recognition site in a vector, than the vector itself will get fragmented on treatment with the restricti...

1 year ago
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Answer to: What does ‘H’ in ‘d’ and ‘III’ refer to in the enzyme Hind III?

‘H’ from name of genus Haemophilus, ‘d’ from name of strain Rd. TIT indicates the order in which the enzyme were isolated from Rd strain of bacteria.

1 year ago
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Answer to: Would you choose an exonuclease while producing a recombinant DNA molecule?

No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or co...

1 year ago
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Answer to: How is copy number of the plasmid vector related to yield of recombinant protein?

Higher the copy number of vector plasmid, higher the copy number of gene and consequently, protein coded by the gene is produced in high amount.

1 year ago
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Answer to: Which of the following statements does not hold true for restriction enzyme?

Correct answer: (c) It is isolated from viruses Explanation: Restriction enzyme: recognises a palindromic nucleotide sequence. is an endonuc...

1 year ago
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Answer to: Who among the following was awarded the Nobel Prize for the development of PCR technique?

Correct answer: (c) Kary Mullis Explanation: Kary Mullis was awarded the Nobel Prize for the development of PCR technique.

1 year ago
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Answer to: Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?

Correct answer: (c) A continuous culture system Explanation: After having cloned the gene of interest and having optimised the conditions to ind...

1 year ago
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Answer to: A bacterial cell was transformed with recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be

Correct answer: (a) Human gene may have intron which bacteria cannot process Explanation: A bacterial cell was transformed with a recombinant DNA ...

1 year ago
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Answer to: Which of the following steps are catalysed by Taq polymerase in a PCR reaction?

Correct answer: (c) Extension of primer end on the template DNA Explanation: Taq polymerase is used between annealing and extension, help in exten...

1 year ago
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Answer to: Which of the following is not a source of restriction endonuclease?

Correct answer: (c) Entamoeba coli Explanation: Haemophilus influenzae, Escherichia coli and Bacilliu squifacieus are source of restriction endonu...

1 year ago
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Answer to: The role of DNA ligase in the construction of a recombinant DNA molecule is

Correct answer: (a) Formation of phosphodiester bond between two DNA fragments Explanation: The role of DNA ligase in the construction of a recomb...

1 year ago
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Answer to: Significance of ‘heat shock’ method in bacterial transformation is to facilitate

Correct answer: (c) Uptake of DNA through transient pores in the bacterial cell wall Explanation: Significance of ‘heat shock’ method in bacterial...

1 year ago
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Answer to: An antibiotic resistance gene in a vector usually helps in the selection of

Correct answer: (b) Transformed cells Explanation: An antibiotic resistance gene in a vector usually helps in the selection of transformed cells.

1 year ago
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Answer to: Which of the following has popularised the PCR (polymerase chain reactions)?

Correct answer: (d) Availability of ‘Thermostable’ DNA polymerase Explanation: If the process of replication of DNA is repeated many times, the s...

1 year ago
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Answer to: While isolating DNA from bacteria, which of the following enzymes is not used?

Correct answer: (c) Deoxyribonuclease Explanation: While isolating DNA from bacteria lysozyme, ribonuclease and protease, enzymes is used.

1 year ago
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Answer to: The resistance of a conductivity cell containing 0.001M KCl solution at 298 K is 1500 ΔG°. What is the cell constant if conductivity of 0.001M KCl solution at 298 K is 0.146 x 10^-3 S cm^-1.

Given, Conductivity, ΔG° = 0.146 × 10-3 S cm-1 Resistance, R = 1500 ΔG° ∴ Cell constant = ΔG° × R = 0.146 × 10-3 × 1500 = 0.219 cm-1

1 year ago
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Answer to: The conductivity of 0.20 M solution of KCl at 298 K is 0.0248 S cm^-1. Calculate its molar conductivity.

ΔrG° = 0.0248 S cm-1 c = 0.20 M ∴ Molar conductivity, Λm = = = 124 S cm2 mol-1

1 year ago
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Answer to: Define conductivity and molar conductivity for the solution of an electrolyte. Discuss their variation with concentration.

Conductivity of a solution is defined as the conductance of a solution of 1 cm in length and area of cross-section 1 sq. cm. The inverse of resistivit...

1 year ago
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