Last seen: Jan 19, 2022
Role of proteases is to degrade the proteins present inside a cell (from which DNA is being isolated). If the proteins are not removed from DNA prepar...
Competent means bacterial cells, on treatment with CaCl2, are made capable of taking up foreign DNA.
If the restriction enzymes have more than one recognition site in a vector, than the vector itself will get fragmented on treatment with the restricti...
‘H’ from name of genus Haemophilus, ‘d’ from name of strain Rd. TIT indicates the order in which the enzyme were isolated from Rd strain of bacteria.
No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or co...
Higher the copy number of vector plasmid, higher the copy number of gene and consequently, protein coded by the gene is produced in high amount.
Correct answer: (c) It is isolated from viruses Explanation: Restriction enzyme: recognises a palindromic nucleotide sequence. is an endonuc...
Correct answer: (c) Kary Mullis Explanation: Kary Mullis was awarded the Nobel Prize for the development of PCR technique.
Correct answer: (c) A continuous culture system Explanation: After having cloned the gene of interest and having optimised the conditions to ind...
Correct answer: (a) Human gene may have intron which bacteria cannot process Explanation: A bacterial cell was transformed with a recombinant DNA ...
Correct answer: (c) Extension of primer end on the template DNA Explanation: Taq polymerase is used between annealing and extension, help in exten...
Correct answer: (c) Entamoeba coli Explanation: Haemophilus influenzae, Escherichia coli and Bacilliu squifacieus are source of restriction endonu...
Correct answer: (a) Formation of phosphodiester bond between two DNA fragments Explanation: The role of DNA ligase in the construction of a recomb...
Correct answer: (c) Uptake of DNA through transient pores in the bacterial cell wall Explanation: Significance of ‘heat shock’ method in bacterial...
Correct answer: (b) Transformed cells Explanation: An antibiotic resistance gene in a vector usually helps in the selection of transformed cells.
Correct answer: (d) Availability of ‘Thermostable’ DNA polymerase Explanation: If the process of replication of DNA is repeated many times, the s...
Correct answer: (c) Deoxyribonuclease Explanation: While isolating DNA from bacteria lysozyme, ribonuclease and protease, enzymes is used.
Given, Conductivity, ΔG° = 0.146 × 10-3 S cm-1 Resistance, R = 1500 ΔG° ∴ Cell constant = ΔG° × R = 0.146 × 10-3 × 1500 = 0.219 cm-1
ΔrG° = 0.0248 S cm-1 c = 0.20 M ∴ Molar conductivity, Λm = = = 124 S cm2 mol-1
Conductivity of a solution is defined as the conductance of a solution of 1 cm in length and area of cross-section 1 sq. cm. The inverse of resistivit...