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During in vitro synthesis of DNA, a researcher used 2′, 3′ - dideoxy cytidine triphosphate as raw nucleotide in place of 2′-deoxycytidine. What would be the consequence?

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During in vitro synthesis of DNA, a researcher used 2′, 3′ - dideoxy cytidine triphosphate as raw nucleotide in place of 2′-deoxycytidine. What would be the consequence?

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Further polymerisation would not occur, as the 3′-OH on sugar is not there to add a new nucleotide for forming ester bond.

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