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18/01/2022 6:18 pm
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Would you choose an exonuclease while producing a recombinant DNA molecule?
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18/01/2022 6:19 pm
No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or completely degrade the DNA fragment containing the gene of interest, and the circular plasmid (vector) will not get cut as it lacks free ends.