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17/01/2022 7:52 pm
Topic starter
Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?
(a) DNA can be seen in visible light
(b) DNA can be seen without staining in visible light
(c) Ethidium bromide stained DNA can be seen in visible light
(d) Ethidium bromide stained DNA can be seen under exposure to UV light
1 Answer
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17/01/2022 7:53 pm
Correct answer: (d) Ethidium bromide stained DNA can be seen under exposure to UV light
Explanation:
The separated DNA fragments can he visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to ultraviolet radiation (we cannot see pure DNA fragments in the visible light and without staining).