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18/01/2022 5:52 pm
Topic starter
Which of the following has popularised the PCR (polymerase chain reactions)?
(a) Easy availability of DNA template
(b) Availability of synthetic primers
(c) Availability of cheap deoxyribonucleotides
(d) Availability of ‘Thermostable’ DNA polymerase
1 Answer
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18/01/2022 5:54 pm
Correct answer: (d) Availability of ‘Thermostable’ DNA polymerase
Explanation:
If the process of replication of DNA is repeated many times, the segment of DNA can be amplified to approximately billion times i.e., 1 billion copies are made. Such repeated amplification is achieved by the use of a thermostable DNA polymerase (isolated from a thermophilic bacterium Thermus aquaticus), which is active during the high temperature induced denaturation of double stranded DNA.