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18/01/2022 8:49 pm
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Describe the role of CaCl2 in the preparation of competent cells.
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18/01/2022 8:50 pm
CaCl2 is known to increase the efficiency of DNA uptake to produce transformed bacterial cells. The divalent Ca2+ ions supposedly create transient pores on the bacterial cell wall by which the entry of foreign DNA is facilitated into the bacterial cells.