Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.
Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.
Palindromes in DNA are base pair sequences that are the same when read forward (left to right) or backward (right to left) from a central axis of symmetry. For example, the following sequences read the same on the two strands in the 5′ → 3′ direction as well as 3′ → 5′ direction.
1. 5′ – G – G – A – T – C – C – 3′
3′ – C – C – T – A – G – G – 5′
2. 5′ – G – A – A – T – T – C – 3′
5′ – C – T – T – A – A – G – 5′
3. 5′ – A – A – G – C – T – T – 3′
3′ – T – T – C – G – A – A – 5′
4. 5′ – G – T – C – G – A – C – 3′
3′ – C – A – G – C – T – G – 5′
5. 5′ – A – C – T – A – G – T – 3′
3′ – T – G – A – T – C – A – 5′
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Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
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Describe the role of Agrobacterium tumefacient in transforming a plant cell.
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For selection of recombinants, insertional inactivation of antibiotic marker has been superseded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
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Name the regions marked A, B and C.
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Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
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