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Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system. 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes Describe the role of Agrobacterium tumefacient in transforming a plant cell. 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes For selection of recombinants, insertional inactivation of antibiotic marker has been superseded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons. 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes Name the regions marked A, B and C. 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below. 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing? 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes Describe the role of CaCl2 in the preparation of competent cells. 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason? 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment? 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes How does one visualise DNA on an agarose gel? 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain. 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e. bacterial transformation? 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct? 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes What is meant by gene cloning? 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes What modification is done on the Ti plasmid of Agrobacterium tumefacient to convert it into a cloning vector? 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions? 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes Name a recombinant vaccine that is currently being used in vaccination program. 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process? 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes What is the significance of adding proteases at the time of isolation of genetic material (DNA)? 3 years ago Shilpi98 class 12 cbse ncert exemplar biotechnology principles processes Page 1 / 5 1 2 3 4 5 Next Share: Super Globals Requests: Server: Options and Features route: community use_home_url: url: https://eanshub.com/community/ pageid:187 default_groupid: 3 2022 How Can We Help? Δ Home About Us Contact Us Privacy Policy Term of Use Copyright © 2020 Eanshub All Rights Reserved
