Notifications
Clear all
0
18/01/2022 7:02 pm
Topic starter
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e. bacterial transformation?
1 Answer
0
18/01/2022 7:03 pm
The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which removes nucleotides from the free ends of DNA.