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Shilpi98
@shilpi98
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Joined: Dec 29, 2021
Last seen: Mar 10, 2022
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Topics: 2165 / Replies: 0
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Topic
Describe the role of CaCl2 in the preparation of competent cells.
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 125
Topic
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 148
Topic
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 171
Topic
How does one visualise DNA on an agarose gel?
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 224
Topic
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 182
Topic
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’.
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 117
Topic
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e. bacterial transformation?
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 124
Topic
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 103
Topic
What is meant by gene cloning?
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 94
Topic
What modification is done on the Ti plasmid of Agrobacterium tumefacient to convert it into a cloning vector?
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 152
Topic
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 246
Topic
Name a recombinant vaccine that is currently being used in vaccination program.
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 117
Topic
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 193
Topic
Bacillus thuringiensis (Bt) strains have been used for designing novel:
4 years ago
Forum
Microbes in Human Welfare
Replies: 0
Views: 229
Topic
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 177
Topic
What does ‘competent’ refer to in competent cells used in transformation experiments?
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 96
Topic
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 75
Topic
What does ‘H’ in ‘d’ and ‘III’ refer to in the enzyme Hind III?
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 145
Topic
Would you choose an exonuclease while producing a recombinant DNA molecule?
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 104
Topic
How is copy number of the plasmid vector related to yield of recombinant protein?
4 years ago
Forum
Biotechnology: Principles and Processes
Replies: 0
Views: 87
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