Last seen: Dec 29, 2021
Since plasmids can replicate within the bacterial cell independently of the genomic DNA, any alien DNA ligated to it will also multiply, i.e. it is us...
(i) Cloning vectors are used to make multiple copies of the desired DNA/ gene. (ii) They are used to transfer the gene of interest to the host cell...
(i) Engineered vectors facilitate easy linking of foreign DNA. (ii) They help in selection of recombinants.
Plasmids and bacteriophages.
Centrifugation is the technique. Centrifuge is the instrument.
The gel is stained by ethidium bromide, to view the separated DNA bands when exposed to UV light.
DNA-fragments are negatively charged and hence, move towards the anode.
Agarose; it provides the sieving effect for the DNA to resolve according to their size.
Gel electrophoresis.
R stands for RY 13, the strain of E.coli bacterium.
They act as molecular scissors to cut DNA at specific locations.
One of them (exonucleases) added methyl groups to DNA. The other (endonucleases) cut the DNA at specific points within the DNA.
Restriction endonuclease and DNA ligase.
An antibiotic-resistance gene and the plasmid of Salmonella typhimurium.
Origin of replication (Ori).
The alien DNA has to be linked to a specific sequence of DNA, called origin of replication, on the chromosome; this origin of replication is responsib...
Gene cloning refers to the process in which a number of identical copies of a gene of interest are made by introducing the gene into an appropriate ho...