Last seen: Dec 29, 2021
Correct answer: (a) [M4L-6T7] Explanation: Dimensional formulae for given quantities G[M-1L3T-2] E [ML2T-2] M[M] L[ML2T-1] ∴ [G-2M2E-3L3...
Correct answer: (a) 0.2 Explanation: In steady state, energy stored in inductor = 64J .......(1) Power consumed in steady state, I2R = 640J...
It is the source of thermostable DNA- polymerase enzyme.
Lysozymes for bacterial cells. Chitinase for fungal cells.
Proteins are removed by using proteases. RNAs are removed by using ribonucleases (RNases).
The bacterial cell has to be treated with lysozyme, to digest the cell wall to release DNA.
Cellulase is used to digest the cell wall (cellulose) of plant cells; animal cells have no cell wall and hence, it is not needed.
The cells are bombarded with high velocity micro-particles of gold or tungsten, coated with the alien DNA.
Agrobacterium tumefaciens.
Retroviruses are disarmed (diseases-causing gene is removed/inactivated) and used as vectors to deliver the recombinant/alien DNA into animal cells.
When it infects a plant cell, it delivers a part of its DNA, called ‘T-DNA’ (or Tumour- inducing (Ti) plasmid) into the plant cell and transforms it i...
When it infects a plant cell, it delivers a part of its DNA, called ‘T-DNA’ (or Tumour- inducing (Ti) plasmid) into the plant cell and transforms it i...
The transformant loses ampicillin-resistance.
The transformant loses the tetracycline- resistance.
In the absence of a selectable marker, it will not be possible to identify the recombinants from the non-recombinants.
Ampicillin-resistance gene and tetracyclin- resistance gene.
It is because the normal E. coli cells do not have resistance to any such antibiotics.
It helps in identifying the recombinants from the non-recombinants.