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How did Hershey and Chase differentiate between DNA and protein in their experiment while proving that DNA is the genetic material?

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How did Hershey and Chase differentiate between DNA and protein in their experiment while proving that DNA is the genetic material?

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They raised 2 types of bacteriophages

  • On radioactive phosphorous (32P)
  • On radioactive sulphur (35S).

35S gets into protein and 32P into DNA When both bacteriophages infected bacteria differently and by shaking them, the viral protein coat was separated.

After raising these bacteria it was found that those infected with 32P bacteriophage → radioactivity were found. But with 35S → no radioactivity was found.

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