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Explant is required to be disinfected before placing in culture. This is done by

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Explant is required to be disinfected before placing in culture. This is done by

(a) autoclaving

(b) ultra-violet rays

(c) Clorox or hypochlorite

(d) X-rays

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(c) Clorox or hypochlorite

Explanation:

Before transferring on the culture medium, the explant is first of all disinfected by surface sterilization using clorax water, sodium or calcium hypochlorite solution or methiolate. Too much care must be taken in this operation so that the cells do not die.

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